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mi RN ome of I talian L arge W hite pig subcutaneous fat tissue: new mi RNA s, isomi R s and mo RNA s
Author(s) -
Gaffo Enrico,
Zambonelli Paolo,
Bisognin Andrea,
Bortoluzzi Stefania,
Davoli Roberta
Publication year - 2014
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12192
Subject(s) - rna , biology , messenger rna , gene expression , adipose tissue , microrna , microbiology and biotechnology , gene , biochemistry
Summary Small RNA s, such as micro‐ RNA s (mi RNA s), are decisive regulators of gene expression, and they could determine adipose tissue traits. A better knowledge of porcine fat genomics is relevant given that the pig is a biomedical model for metabolic and cardiovascular human pathologies. Adipose tissue is particularly important for the meat industry. We explored the mi RN ome of two adult I talian L arge W hite pig backfat samples by Illumina RNA ‐ S eq. Using custom bioinformatic methods, the expressed mi RNA s were identified and quantified and the nucleotide sequence variability of mi RNA isoforms were analysed. We detected 222 known mi RNA s, 68 new mi RNA s and 17 miRNA‐offset RNA s (mo RNA s) expressed from known hairpins, and 312 new mi RNA s expressed from 253 new hairpins. Porcine transcripts targeted by the most expressed mi RNA s were predicted, showing that these mi RNA s may have an impact on W nt, insulin signalling and axon guidance pathways. The expression of five small RNA s, including mo RNA ssc‐5′‐mo R ‐21 and a mi RNA from a new hairpin, was validated by a q RT ‐ PCR assay, thus confirming the robustness of our results. The depicted mi RN ome complexity suggests that quantitative and qualitative features of mi RNA s and non‐canonical products of their precursors are worthy of further investigation to clarify their roles in the adipose tissue biology.