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Quantitative trait loci mapping for conjugated linoleic acid, vaccenic acid and ∆ 9 ‐desaturase in Italian Brown Swiss dairy cattle using selective DNA pooling
Author(s) -
Strillacci M. G.,
Frigo E.,
Canavesi F.,
Ungar Y.,
Schiavini F.,
Zaniboni L.,
Reghenzani L.,
Cozzi M. C.,
Samoré A. B.,
Kashi Y.,
Shimoni E.,
TalStein R.,
Soller M.,
Lipkin E.,
Bagnato A.
Publication year - 2014
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12174
Subject(s) - biology , sire , vaccenic acid , conjugated linoleic acid , linoleic acid , quantitative trait locus , single nucleotide polymorphism , fatty acid desaturase , genetics , fatty acid , snp , dairy cattle , gene , polyunsaturated fatty acid , zoology , biochemistry , genotype
Summary A selective DNA pooling approach was applied to identify QTL for conjugated linoleic acid ( CLA ), vaccenic acid ( VA ) and Δ 9 ‐desaturase (D9D) milk content in Italian Brown Swiss dairy cattle. Milk samples from 60 animals with higher values (after correction for environmental factors) and 60 animals with lower values for each of these traits from each of five half‐sib families were pooled separately. The pools were genotyped using the Illumina Bovine SNP 50 BeadChip. Sire allele frequencies were compared between high and low tails at the sire and marker level for SNP s for which the sires were heterozygous. An r procedure was implemented to perform data analysis in a selective DNA pooling design. A correction for multiple tests was applied using the proportion of false positives among all test results. BTA 19 showed the largest number of markers in association with CLA . Associations between SNP s and the VA and Δ 9 ‐desaturase traits were found on several chromosomes. A bioinformatics survey identified genes with an important role in pathways for milk fat and fatty acids metabolism within 1 Mb of SNP markers associated with fatty acids contents.