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Identification of species‐specific novel transcripts in pig reproductive tissues using RNA ‐seq
Author(s) -
Du Z.Q.,
Eisley C. J.,
Onteru S. K.,
Madsen O.,
Groenen M. A. M.,
Ross J. W.,
Rothschild M. F.
Publication year - 2014
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12124
Subject(s) - biology , rna seq , identification (biology) , rna , transcriptome , computational biology , gene , genetics , gene expression , ecology
Summary Although structural properties of the porcine reproductive system are shared by many placental mammals, some combination of these properties is unique to pigs. To explore whether genomic elements specific to pigs could potentially underlie this uniqueness, we made the first step to identify novel transcripts in two representative pig reproductive tissues by the technique of massively parallel sequencing. To automate the whole process, we built a computational pipeline, which can also be easily extended for similar studies in other species. In total, 5516 and 9061 novel transcripts were found, and 159 and 252 novel transcripts appear to be specific to pigs for the placenta and testis respectively. Furthermore, these novel transcripts were found to be enriched in quantitative trait loci ( QTL ) regions for reproduction traits in pigs. We validated eight of these novel transcripts by quantitative real‐time PCR . With respect to their genomic organization and their functional relationship to reproduction, these transcripts need to be further validated and explored in various pig breeds to better comprehend the relevant aspects of pig physiology that contribute to reproductive performance.