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Identification and genetic effect of a variable duplication in the promoter region of the cattle ADIPOQ gene
Author(s) -
Zhang L.,
Yang M.,
Li C.,
Xu Y.,
Sun J.,
Lei C.,
Lan X.,
Zhang C.,
Chen H.
Publication year - 2014
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12112
Subject(s) - biology , gene duplication , gene , quantitative trait locus , marbled meat , locus (genetics) , genetics , microbiology and biotechnology
Summary In cattle, the ADIPOQ gene is located in the vicinity of the quantitative trait locus ( QTL ) affecting marbling, the ribeye muscle area and fat thickness on BTA 1. In our study, a novel variable duplication ( NW_003103812.1 :g.9232067_9232133dup) in the bovine ADIPOQ promoter region was identified and genotyped in seven Chinese cattle breeds. Using a reporter assay, we demonstrated that g.9232067_9232133dup decreased the basal transcriptional activity of the ADIPOQ gene in the 3T3‐L1 and C2C12 cells. Furthermore, g.9232067_9232133dup suppressed the m RNA expression of the gene in adipose and muscle tissues. An association analysis indicated that the incremental variable duplication was associated with body measurements.