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Significant association between SNP s in the superoxide dismutase 3, extracellular ( SOD 3 ) gene and resistance to A eromonas hydrophila in the freshwater mussel H yriopsis cumingii
Author(s) -
Wang G. L.,
Li X. L.,
Li J. L.
Publication year - 2013
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12059
Subject(s) - biology , locus (genetics) , linkage disequilibrium , genetics , superoxide dismutase , microbiology and biotechnology , single nucleotide polymorphism , allele , peptide sequence , genotype , gene , sequence analysis , biochemistry , enzyme
Summary Extracellular superoxide dismutase (SOD3) is a major antioxidant enzyme that protects organs from damage by reactive oxygen species (ROS). In this study, the SOD3 gene was identified and characterized from the freshwater mussel H yriopsis cumingii (Hc ‐SOD3 ). The cDNA sequence consists of 763 bp, encoding a protein of 208 amino acids. The amino acid sequence possesses two CuZnSOD signature sequences, and amino acids required for binding of Cu (His‐93, ‐95, ‐110 and ‐169) and Zn (His‐110, ‐118, ‐129 and Asp‐132) were conserved in Hc ‐SOD3 . The Hc ‐SOD3 genomic sequence was 9165 bp in length, containing four exons and three introns. Eighteen single nucleotide polymorphisms were detected in the Hc‐ SOD3 gene from resistant stock (RS) and susceptible stock (SS) of H . cumingii to A eromonas hydrophila . The genotype and allele distribution were examined in resistant and susceptible stocks. Among them, a C/G substitution at the g.7994C>G locus and G/C substitution at the g.8087G>C locus were significantly associated with resistance/susceptibility of H . cumingii to A . hydrophila, both in genotype ( P = 0.017, P = 0.004 respectively) and allele frequency ( P = 0.021, P = 0.006 respectively). Linkage disequilibrium analysis revealed that g.7994C>G, g.8001A>G, g.8035G>A, g.8087G>C and g.8191T>A were in linkage disequilibrium. The results suggest that the two polymorphic loci, g.7994C>G and g.8087G>C, could be potential genetic markers for future molecular selection of strains that are resistant to diseases.