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Effects of hydroxy‐delta‐5‐steroid dehydrogenase, 3 beta‐ and steroid delta‐isomerase 1 polymorphisms on fat androstenone level and gene expression in D uroc pigs
Author(s) -
Kim J. M.,
Ahn J. H.,
Lim K. S.,
Lee E. A.,
Chun T.,
Hong K. C.
Publication year - 2013
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12041
Subject(s) - androstenone , boar taint , biology , single nucleotide polymorphism , genotype , endocrinology , adipose tissue , medicine , skatole , intramuscular fat , boar , gene , genetics , biochemistry , indole test , sperm
Summary A high level of androstenone in porcine adipose tissue is a major factor contributing to boar taint. P orcine hydroxy‐delta‐5‐steroid dehydrogenase, 3 beta‐ and steroid delta‐isomerase 1 ( 3β‐ HSD , also known as HSD3B1 ) plays a key role in the hepatic metabolism that catalyzes androstenone to β‐androstenol. Therefore, 3β‐ HSD is a candidate gene for boar taint. This study aimed to investigate functional 3β‐ HSD polymorphisms in D uroc pigs. We found eight single nucleotide polymorphisms ( SNP s) in the full‐length porcine 3β‐ HSD . Four of the SNP s had restriction enzyme sites, and we genotyped them in 147 uncastrated male D uroc pigs using a polymerase chain reaction–restriction fragment length polymorphism method. Pigs with the GG genotype at the g.165262G>A locus ( SNP 5) had significantly lower androstenone levels than did those with other genotypes ( P = 0.030). SNP 5 also was associated with differences in 3β‐ HSD mRNA levels: pigs with the GG genotype had higher levels than those with other genotypes ( P = 0.019). The SNP 5 polymorphism could affect the hepatic catabolism of androstenone and consequently impact androstenone accumulation in the adipose tissue. Therefore, SNP 5 in the 3β‐ HSD of D uroc pigs could be a useful selective marker for decreasing boar taint.