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Transcriptional profiling and mi RNA ‐dependent regulatory network analysis of longissimus dorsi muscle during prenatal and adult stages in two distinct pig breeds
Author(s) -
Siengdee P.,
Trakooljul N.,
Murani E.,
Schwerin M.,
Wimmers K.,
Ponsuksili S.
Publication year - 2013
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12032
Subject(s) - biology , microrna , skeletal muscle , gene expression profiling , microarray , transcriptome , microarray analysis techniques , genetics , computational biology , gene expression , gene , bioinformatics , andrology , microbiology and biotechnology , anatomy , medicine
Summary MicroRNAs (miRNAs) and mRNA s establish a complex regulatory network influencing diverse biological pathways including muscle development and growth. Elucidating miRNA‐dependent regulatory networks involved in muscle development could provide additional insights into muscle traits largely predefined during prenatal development. The present study aimed to determine differentially expressed transcripts and functional miRNA– mRNA relationships associated with different stages of skeletal muscle development in two pig breeds, German Landrace and Pietrain, distinct in muscle characteristics. A comparative transcriptional profiling of longissimus dorsi muscle tissues from fetuses at 35, 63 and 91 days post‐conception as well as adult pigs (180 days postnatum) was performed using the Affymetrix GeneChip porcine genome microarray. Differential expression patterns were identified to be associated with muscularly developmental stages and breed types. The integration of miRNA expression data and ingenuity pathways analysis (ipa) pathway analysis revealed several miRNA‐dependent regulatory networks related to muscle growth and development. The present results provide insights into muscle biology for further improvement of porcine meat quality.