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Two completely linked polymorphisms in the PPARG transcriptional regulatory region significantly affect gene expression and intramuscular fat deposition in the longissimus dorsi muscle of E rhualian pigs
Author(s) -
Wang H.,
Xiong K.,
Sun W.,
Fu Y.,
Jiang Z.,
Yu D.,
Liu H.,
Chen J.
Publication year - 2013
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12025
Subject(s) - intramuscular fat , peroxisome proliferator activated receptor gamma , biology , single nucleotide polymorphism , longissimus dorsi , haplotype , allele , single strand conformation polymorphism , large white , gene , genotype , microbiology and biotechnology , exon , genetics , zoology , peroxisome proliferator activated receptor
Summary This article is the first to report the significant effect of PPARG promoter polymorphisms on the expression and intramuscular fat ( IMF ) content in the longissimus dorsi ( LD ) muscle of E rhualian pigs. Polymorphic sites for the PPARG upstream transcriptional regulatory region were scanned by direct sequencing of two DNA pools consisting of individuals with significantly different IMF content ( P < 0.05). Two SNP s (c.‐1633C>T and c.‐1572G>A) were identified, with different allele distribution between these two groups. A total of 66 E rhualian pigs and 24 Y orkshire × L arge W hite (Y × L) pigs were genotyped for these two SNP s using PCR ‐ SSCP (single‐strand conformation polymorphism), which exhibited that only the T‐A and C‐G haplotypes existed. The T‐A type promoter was found to have higher activity than G‐C type promoter for PPARG gene transcription in the induced preadipocytes using luciferase assay. In addition, the frequency of the T‐A type in E rhualian pigs was much higher than that in the Y × L pigs (35.6% vs. 8.3%), and in fact, no T‐A / T‐A samples were found in Y × L pigs. Therefore, we conclude that the T‐A haplotype of PPARG is a desirable form that might contribute to the relatively higher IMF content in E rhualian pigs.