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Genetic diversity and differentiation of A canthoscelides obtectus S ay ( C oleoptera: B ruchidae) populations in C hina
Author(s) -
Duan Canxing,
Zhu Zhendong,
Li Wanchang,
Bao Shiying,
Wang Xiaoming
Publication year - 2017
Publication title -
agricultural and forest entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.755
H-Index - 55
eISSN - 1461-9563
pISSN - 1461-9555
DOI - 10.1111/afe.12187
Subject(s) - biology , fixation index , analysis of molecular variance , genetic diversity , genetic variation , locus (genetics) , allele , loss of heterozygosity , gene flow , microsatellite , genetic distance , genetics , botany , population , gene , demography , sociology
Bean weevil A canthoscelides obtectus S ay is one of the most destructive pests of bean seeds. Using 10 microsatellite loci, we studied the genetic diversity and differentiation of 13 geographical populations. A total of 79 alleles were detected, with an average of 7.900 alleles per locus. The gene flow and genetic differentiation rate values at the 10 loci varied from 0.376 to 1.560 and 13.8–39.9%, with averages of 0.774% and 24.4%, respectively. Among the 13 populations, the effective number of alleles and observed heterozygosity ranged from 1.865 to 2.848 and 0.457 to 0.646, respectively. Shannon's information index ranged from 0.684 to 1.104. In all comparisons, the fixation index ( F ST ) values ranged from 0.021 to 0.463, with a total F ST value of 0.228 among the 13 populations, indicating a moderate level of genetic differentiation among these populations. Analysis of molecular variance revealed that the genetic variation within populations accounted for 67.7% of the total genetic variation. Using the unweighted pair group method with arithmetic mean cluster analysis, 13 populations were clustered into three distinct genetic groups. The pattern of the three concentrated groups from principal component analysis showed a similar result to cluster, although some individuals interpenetrated with one another.