Premium
Biochemistry and molecular characterisation of chlorpyrifos resistance in field strains of the white‐backed planthopper S ogatella furcifera (Hemiptera: Delphacidae)
Author(s) -
He Ming,
Ai ZhenXian,
Jiang ZiQiong,
Long YuNing,
Zhang YueLiang,
He Peng
Publication year - 2015
Publication title -
austral entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.502
H-Index - 39
eISSN - 2052-1758
pISSN - 2052-174X
DOI - 10.1111/aen.12140
Subject(s) - delphacidae , biology , chlorpyrifos , acetylcholinesterase , nymph , organophosphate , brown planthopper , complementary dna , gene , genetics , microbiology and biotechnology , pest analysis , biochemistry , botany , enzyme , pesticide , homoptera , agronomy
Acetylcholinesterase ( AChE ) is the molecular target of organophosphate ( OP ) and carbamate ( CB ) insecticides. The white‐backed planthopper, S ogatella furcifera ( H orváth, 1899), is a notorious rice pest in A sia and exhibits high resistance against a specific OP pesticide known as chlorpyrifos. To date, there has not yet been a study on the molecular mechanisms that confer resistance to this pesticide in S . furcifera . In this study, we cloned the full‐length cDNA of AChE type 1 and type 2 genes from S . furcifera ( SfAChE1 and SfAChE2 ). Both genes appear to be functional and are composed of the classic AChE domains. An analysis of the gene family revealed that SfAChE1 and SfAChE2 cluster into in the insect AChE 1 and AChE 2 clades, respectively. We first performed qPCR experiments to detect AChE expression patterns in distinct developmental stages and tissues. Our results revealed that SfAChE1 is more highly expressed than SfAChE2 . Both genes were most highly expressed during the first instar nymph stage, and then expressed at a decreasing rate until the end of the fifth instar nymph stage. Both SfAChE1 and SfAChE2 were more highly expressed than in other tested tissues. Furthermore, we investigated the association between AChE genes and chlorpyrifos resistance and found that N anjing ( NJ ‐ R ) is a chlorpyrifos‐resistant strain with high frequencies of a S er298 P ro mutation in SfAChE 1. Conversely, the chlorpyrifos‐susceptible G uangdong ( GD ‐ S ) strain did not carry this mutation.