Premium
Identification of incursions of C ulicoides L atreille species ( D iptera: C eratopogonidae) in A ustralasia using morphological techniques and DNA barcoding
Author(s) -
Bellis Glenn A,
Gopurenko David,
Cookson Beth,
Postle Anthony C,
Halling Luke,
Harris Nick,
Yanase Tohru,
Mitchell Andrew
Publication year - 2015
Publication title -
austral entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.502
H-Index - 39
eISSN - 2052-1758
pISSN - 2052-174X
DOI - 10.1111/aen.12131
Subject(s) - ceratopogonidae , dna barcoding , biology , culicoides , zoology , mitochondrial dna , new guinea , cytochrome c oxidase subunit i , ecology , genetics , ethnology , history , gene
Light trap surveillance across northern A ustralia and P apua N ew G uinea ( PNG ) has detected the presence of several O riental species of C ulicoides not previously reported from those countries and which appear to have arrived in recent times. D etections of C . nudipalpis D elfinado in Western A ustralia, C . flavipunctatus K itaoka and C . palpifer D as G upta and G hosh in the N orthern T erritory and of C . flavipunctatus , C . fulvus S en and D as G upta and C . orientalis M acfie in Q ueensland ( Q ld) provide evidence of multiple pathways for incursions of biting midges into northern A ustralia. Of these, only C . fulvus appears to have established. Additionally, three species, C . fulvus , C . wadai Kitaoka and C . brevipalpis D elfinado, are newly reported from PNG and all appear to be well established. The arrival in PNG of C . fulvus and C . brevipalpis , both not previously reported from Q ld, suggests that pathways exist for the entry of O riental insects into N ew G uinea directly from A sia, rather than via A ustralia. Molecular analyses using DNA barcodes (partial mitochondrial cytochrome oxidase subunit one sequences) confirmed morphological identification of specimens and additionally provided strong evidence relating to the source of these incursions. At least two of these species are vectors of important livestock viruses and are likely to impact on the epidemiology of these viruses as they continue to disperse.