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The influence of irrigation solutions in the inorganic and organic radicular dentine composition
Author(s) -
Barón Marta,
Morales Victoria,
Fuentes Mª Victoria,
Linares María,
Escribano Nuria,
Ceballos Laura
Publication year - 2020
Publication title -
australian endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.703
H-Index - 34
eISSN - 1747-4477
pISSN - 1329-1947
DOI - 10.1111/aej.12395
Subject(s) - radicular cyst , composition (language) , irrigation , dentistry , chemistry , materials science , medicine , agronomy , biology , pathology , philosophy , linguistics , cyst
The aim of this study was to analyse changes in radicular dentine composition after different irrigation regimes using attenuated total reflectance Fourier‐transform infrared spectroscopy (ATR‐FTIR). Dentine slices from human single‐root teeth were immersed in: (i) 5.25% sodium hypochlorite (NaOCl) for 1, 5 and 20 min; (ii) 17% EDTA for 1 min; and (iii) 5.25% NaOCl for 20 min, and 17% EDTA and 5.25% NaOCl, both for 1 min. Carbonate/mineral, Amide I/mineral and Amide III/CH 2 ratios were determined using ATR‐FTIR before and after immersion in the irrigation solutions tested. Results showed that 5.25% NaOCl significantly decreased carbonate/mineral, Amide I/mineral and Amide III/CH 2 ratios ( P < 0.05). Application of 17% EDTA produced no change in carbonate/mineral ratio ( P = 0.120), while an increase in Amide I/mineral in apical third ( P = 0.002) and Amide III/CH 2 ( P < 0.001) was observed. The combination of NaOCl, EDTA and NaOCl increased carbonate/mineral ratio in coronal third ( P = 0.037), and Amide I/mineral ( P = 0.003) and Amide III/CH 2 ( P = 0.001) ratios. In conclusion, irrigation solutions tested significantly affected radicular dentine composition.