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Changes of CD 90 expression and immunoreactive cell localisation in rat dental pulp after cavity preparation
Author(s) -
Sano Yousuke,
Sugiuchi Akina,
Mitomo Keisuke,
Yanagisawa Akihide,
Kambe Ryo,
Furusawa Masahiro,
Muramatsu Takashi
Publication year - 2019
Publication title -
australian endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.703
H-Index - 34
eISSN - 1747-4477
pISSN - 1329-1947
DOI - 10.1111/aej.12307
Subject(s) - odontoblast , immunohistochemistry , pulp (tooth) , chemistry , immunofluorescence , molar , microbiology and biotechnology , pathology , dentistry , biology , antibody , medicine , immunology
Abstract CD 90 expression and immunoreactive cell localisation in rat dental pulp cells after cavity preparation was investigated. Cavity preparation was performed on the maxillary first molar of 8‐week‐old Wistar rats ( n = 36), and immunohistochemistry and quantitative real‐time PCR were performed. CD 90‐immunoreactivity was observed among subodontoblastic cells in the control group. One day after cavity preparation, the CD 90‐immunoreactivity disappeared under the cavity area. While CD 90‐immunoreactivity was faint after 3 days, the re‐arrangement of odontoblasts was detected in contact with dentine. After 5 days, the odontoblasts were observed beneath the dentine, and CD 90‐immunoreactive cells were localised under the odontoblast layer. Immunofluorescence showed co‐localisation of CD 90 and nestin was detected after 3 days. After 5 days, CD 90‐immunoreactivity increased at the subodontoblastic layer. mRNA expression of CD 90 and DSPP decreased after cavity preparation, and gradually recovered ( P < 0.01). These results suggest that CD 90‐immunoreactive cells in the subodontoblastic layer contribute to regeneration of odontoblast and subodontoblastic layers following cavity preparation.