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Investigation of the regenerative potential of necrotic mature teeth following different revascularisation protocols
Author(s) -
Fahmy Sarah Hossam,
Hassanien Ehab El Sayed,
Nagy Mohamed Mokhtar,
El Batouty Kariem Mostafa,
Mekhemar Mohamed,
Fawzy El Sayed Karim,
Hassanein El Hassan,
Wiltfang Jörg,
Dörfer Christof
Publication year - 2017
Publication title -
australian endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.703
H-Index - 34
eISSN - 1747-4477
pISSN - 1329-1947
DOI - 10.1111/aej.12210
Subject(s) - vimentin , vascularity , dentistry , antibiotics , ciprofloxacin , medicine , chemistry , pathology , immunohistochemistry , biochemistry
Abstract This study aimed to assess the revascularisation potential of necrotic mature teeth in a dog model following different protocols. Periapical infection was induced in 54 mature premolars. Teeth were distributed into seven groups: (1) Double‐antibiotic‐paste/Blood clot, (2) Ciprofloxacin/collagen, (3) Double‐antibiotic‐paste/Collagen, (4) Modified Tri‐antibiotic‐paste /collagen, (5) Ciprofloxacin/Gelfoam, (6) Double‐antibiotic‐paste/Gelfoam, and (7) Modified Triantibiotic‐ paste/Gelfoam. Positive and negative controls included infected and healthy teeth, respectively, ( n = 12 roots/group). Canals were apically shaped to size 0.6 mm then disinfected for 1 month. Intra‐canal bleeding was induced then scaffolds were applied for another month. Teeth and supporting bone were surgically sampled. Tissues were histologically scored and vimentin immuno‐intensity was estimated. Ciprofloxacin and Double‐antibiotic paste/Collagen resulted in significantly better corono‐apical tissue ingrowths, vascularity, cementum formation and significantly lower inflammatory extents ( P < 0.05).These groups also showed significantly higher Vimentin intensities, ( P < 0.05). The applied protocols revascularised necrotic mature canals and reduced inflammation particularly in the Ciprofloxacin/collagen and Double‐antibiotic‐paste/collagen groups.