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Determination of mutagenicity of the precipitate formed by sodium hypochlorite and chlorhexidine using the A mes test
Author(s) -
Patil Pranali,
Aminoshariae Anita,
Harding Jarrod,
Montagnese Thomas A,
Mickel Andre
Publication year - 2016
Publication title -
australian endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.703
H-Index - 34
eISSN - 1747-4477
pISSN - 1329-1947
DOI - 10.1111/aej.12100
Subject(s) - sodium hypochlorite , chlorhexidine , ames test , dimethyl sulfoxide , salmonella , chemistry , nuclear chemistry , hypochlorite , microbiology and biotechnology , dentistry , organic chemistry , medicine , bacteria , biology , genetics
The aim of this study was to determine the direct mutagenic potential of any precipitate formed by combining sodium hypochlorite ( NaOCl ) and chlorhexidine ( CHX ). The precipitates formed by NaOCl and CHX were dissolved in 100% dimethyl sulfoxide and cultured with mutant Salmonella T yphimurium strains. The cells were observed for reverse mutation. The numbers of positive/mutated wells were statistically compared with those in the background plates using the two‐sample proportion independent t ‐test. The precipitates were not found to be significantly more mutagenic than the background plates. Within the limitations of this study, the results suggest that the precipitates formed when sodium hypochlorite and chlorhexidine contact did not show mutagenic (and are therefore carcinogenic) potential.