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Effectiveness of KTP laser versus 980 nm diode laser to kill E nterococcus faecalis in biofilms developed in experimentally infected root canals
Author(s) -
Romeo Umberto,
Palaia Gaspare,
Nardo Alessia,
Tenore Gianluca,
Telesca Vito,
Kornblit Roly,
Del Vecchio Alessandro,
Frioni Alessandra,
Valenti Piera,
Berlutti Francesca
Publication year - 2015
Publication title -
australian endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.703
H-Index - 34
eISSN - 1747-4477
pISSN - 1329-1947
DOI - 10.1111/aej.12057
Subject(s) - enterococcus faecalis , laser , materials science , root canal , diode , dentistry , potassium titanyl phosphate , biofilm , biomedical engineering , optoelectronics , optics , medicine , chemistry , bacteria , escherichia coli , biochemistry , physics , gene , genetics , biology
This study aimed to evaluate the antibacterial action of KTP (potassium‐titanyl‐phosphate) laser irradiations (compared with 980 nm diode laser), associated with conventional endodontic procedures, on E nterococcus faecalis biofilms. Fifty‐six dental roots with single canals were prepared with Ni‐Ti rotary instruments, autoclaved, inoculated with an E . faecalis suspension and incubated for 72 h. They were randomly allocated to control and treatment groups. Laser parameters were as follows: power 2.5 W , Ton 35 ms, Toff 50 ms ( KTP laser); power 2.5 W , T on 30 ms, T off 30 ms (980 nm diode laser). To evaluate the residual bacterial load, B io T imer A ssay was employed. The chemo‐mechanical treatment together with laser irradiations ( KTP and 980 nm diode lasers) achieved a considerable reduction of bacterial load (higher than 96% and 93%, respectively). Regarding both laser systems, comparisons with conventional endodontic procedures (mortality rate of about 67%) revealed statistically highly significant differences ( P ≤ 0.01). This study confirms that laser systems can provide an additional aid in endodontic disinfection.