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Clinical investigation of the effect of calcium hydroxide intracanal dressing on bacterial lipopolysaccharide reduction from infected root canals
Author(s) -
Adl Alireza,
Motamedifar Mohammad,
Shams Mahdi Sedigh,
Mirzaie Ahmad
Publication year - 2015
Publication title -
australian endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.703
H-Index - 34
eISSN - 1747-4477
pISSN - 1329-1947
DOI - 10.1111/aej.12054
Subject(s) - calcium hydroxide , root canal , dentistry , pulp (tooth) , periodontitis , periapical periodontitis , limulus , pulp necrosis , significant difference , medicine , chemistry , biology , paleontology
The purpose of this clinical study was to determine the effect of 7 day intracanal dressing with calcium hydroxide on the amount of bacterial lipopolysaccharide ( LPS ; endotoxin) in human teeth with necrotic and infected pulp and apical periodontitis. Twenty‐five single‐rooted teeth with necrotic pulps and apical periodontitis were selected. Samples were collected before ( S 1), after root canal preparation ( S 2) and after 7 day intracanal dressing with calcium hydroxide ( S 3). The limulus amoebocyte lysate assay was used to quantify LPS . LPS was present in 100% of the root canals before ( S 1), after preparation ( S 2) and after 7 day intracanal dressing ( S 3). A significant reduction, equal to 29.54%, was found after root canal preparation ( P < 0.05). A significant difference (equal to 25.26% reduction) was also detected between S 2 and S 3 ( P < 0.05). Total endotoxin reduction ( S 3 compared with S 1) was found to be 47.34%. Endotoxin concentration of the infected root canals was reduced after root canal preparation and also after 7 days of dressing of canals with calcium hydroxide; however, relatively high values of endotoxin remained in the root canals.