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Efficacy of low‐molecular weight chitosan against Candida albicans biofilm on polymethyl methacrylate resin
Author(s) -
Srimaneepong Viritpon,
Thanamee Thanaporn,
Wattanasirmkit Kamolporn,
Muangsawat Sureeporn,
Matangkasombut Oranart
Publication year - 2021
Publication title -
australian dental journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 71
eISSN - 1834-7819
pISSN - 0045-0421
DOI - 10.1111/adj.12826
Subject(s) - chitosan , candida albicans , cleanser , biofilm , corpus albicans , chemistry , microbiology and biotechnology , chlorhexidine , polymethyl methacrylate , acetic acid , nuclear chemistry , bacteria , biology , medicine , dentistry , organic chemistry , polymer , genetics
Background Candida biofilm is a major cause of denture stomatitis. We aimed to compare the efficacy of low‐molecular‐weight chitosan solutions against Candida albicans biofilm on polymethyl methacrylate (PMMA) resin. Methods Various types of chitosan were tested for anti‐ Candida activity by broth dilution. Two types were selected for further testing on 24‐hour C.albicans biofilm formed on PMMA specimens. Specimens were randomly distributed among experimental groups, including 0.1% and 0.2% acetic acid, 3 and 6 mg/mL of oligomer chitosan and 30 kDa chitosan solutions, effervescent tablet (Polident), and 0.2% chlorhexidine, and immersed for 5 min to 12 h. The viability of C. albicans after cleansing were determined by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assays. Remaining viability was calculated into percentage relative to respective controls and analyzed using ANOVA with Tukey post‐hoc tests. Live/dead fluorescence microscopy was also performed. Results Chitosan solutions had high efficacy against C. albicans biofilm on PMMA. The mean relative viability compared to control after 12‐h immersion was 6.60 ± 4.75% and 12.72 ± 6.96% for 3 and 6 mg/mL oligomer, respectively, and 11.68 ± 4.81% and 18.08 ± 6.20% for 3 and 6 mg/mL 30 kDa chitosan, respectively. Conclusions Low‐molecular‐weight chitosan solution is an effective antifungal denture cleanser that can significantly reduce C. albicans viability in biofilm on PMMA.