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Effect of chronic ethanol consumption in rhesus macaques on the nucleus accumbens core transcriptome
Author(s) -
Walter Nicole,
CerveraJuanes Rita,
Zheng Christina,
Darakjian Priscila,
Lockwood Denesa,
CuzonCarlson Verginia,
Ray Karina,
Fei Suzanne,
Conrad Don,
Searles Robert,
Grant Kathleen,
Hitzemann Robert
Publication year - 2021
Publication title -
addiction biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.445
H-Index - 78
eISSN - 1369-1600
pISSN - 1355-6215
DOI - 10.1111/adb.13021
Subject(s) - nucleus accumbens , transcriptome , biology , gene , false discovery rate , gene expression , genetics , receptor
The nucleus accumbens core (NAcc) has been repeatedly demonstrated to be a key component of the circuitry associated with excessive ethanol consumption. Previous studies have illustrated that in a nonhuman primate (NHP) model of chronic ethanol consumption, there is significant epigenetic remodeling of the NAcc. In the current study, RNA‐Seq was used to examine genome‐wide gene expression in eight each of control, low/binge (LD*), and high/very high (HD*) rhesus macaque drinkers. Using an FDR < 0.05, zero genes were significantly differentially expressed (DE) between LD* and controls, six genes between HD* and LD*, and 734 genes between HD* and controls. Focusing on HD* versus control DE genes, the upregulated genes ( N = 366) were enriched in genes with annotations associated with signal recognition particle (SRP)‐dependent co‐translational protein targeting to membrane (FDR < 3 × 10 −59 ), structural constituent of ribosome (FDR < 3 × 10 −47 ), and ribosomal subunit (FDR < 5 × 10 −48 ). Downregulated genes ( N = 363) were enriched in annotations associated with behavior (FDR < 2 × 10 −4 ), membrane organization (FDR < 1 × 10 −4 ), inorganic cation transmembrane transporter activity (FDR < 2 × 10 −3 ), synapse part (FDR < 4 × 10 −10 ), glutamatergic synapse (FDR < 1 × 10 −6 ), and GABAergic synapse (FDR < 6 × 10 −4 ). Ingenuity Pathway Analysis (IPA) revealed that EIF2 signaling and mTOR pathways were significantly upregulated in HD* animals (FDR < 3 × 10 −33 and <2 × 10 −16 , respectively). Overall, the data supported our working hypothesis; excessive consumption would be associated with transcriptional differences in GABA/glutamate‐related genes.