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Orexin‐1 receptor signalling in the prelimbic cortex and ventral tegmental area regulates cue‐induced reinstatement of ethanol‐seeking in iP rats
Author(s) -
Brown Robyn Mary,
Kim Andrezza K.,
Khoo Shaun YonSeng,
Kim Jee Hyun,
Jupp Bianca,
Lawrence Andrew John
Publication year - 2016
Publication title -
addiction biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.445
H-Index - 78
eISSN - 1369-1600
pISSN - 1355-6215
DOI - 10.1111/adb.12251
Subject(s) - ventral tegmental area , infralimbic cortex , orexin receptor , neuroscience , orexin , prefrontal cortex , lateral hypothalamus , psychology , neuropeptide , medicine , receptor , stimulation , dopamine , cognition , dopaminergic
Orexins (hypocretins) are hypothalamic neuropeptides that innervate the entire neuraxis, including the prelimbic cortex and ventral tegmental area and have been implicated in ethanol‐seeking behaviour. The present study aimed to use the orexin‐1 ( OX 1 ) receptor antagonist SB ‐334867 to examine the role of prelimbic cortex and ventral tegmental area OX 1 receptors in cue‐induced reinstatement of ethanol‐seeking. Ethanol‐preferring rats ( iP ) rats were trained to self‐administer ethanol (10 percent v/v, FR 3) or sucrose (0.2–1 percent w/v, FR 3) in the presence of reward‐associated cues before being implanted with indwelling guide cannulae. Rats then underwent extinction training for 11 days. On test days, rats were given a microinjection of vehicle or SB ‐334867 (3 μ g/side) and presented with reward‐associated cues to precipitate reinstatement. Results show SB ‐334867 infused into the prelimbic cortex attenuated cue‐induced reinstatement of ethanol‐seeking, but not sucrose‐seeking. OX 1 antagonism in the ventral tegmental area also attenuated cue‐induced reinstatement of ethanol‐seeking. These findings suggest that OX 1 receptors located in the prelimbic cortex and ventral tegmental area are part of a circuit driving cue‐mediated ethanol‐seeking behaviour.