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RNA Sequencing Reveals a Comprehensive Circular RNA Expression Profile in a Mouse Model of Alcoholic Liver Disease
Author(s) -
Dou Xiaobing,
Feng Luyan,
Ying Na,
Ding Qinchao,
Song Qing,
Jiang Fusheng,
Wang Cui,
Li Songtao
Publication year - 2020
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/acer.14265
Subject(s) - microrna , biology , circular rna , crosstalk , gene , fold change , rna , gene expression , computational biology , microbiology and biotechnology , genetics , physics , optics
Background and Aims Alcoholic liver disease (ALD) is the most common liver disease and a severe mortality burden in the world. However, ALD is rarely detected at its early stages. Thus, exploration of an early event for ALD may help the prognosis and further therapy of ALD. Several circRNAs were proven as novel molecular biomarkers for the progression of chronic nonalcoholic fatty liver disease. Whether circRNAs are involved explicitly in ALD remains unknown. Methods The expression profile of circRNAs in an ALD mouse model was depicted by circRNA sequencing. The dysregulated circRNAs were verified by quantitative reverse transcription polymerase chain reaction (qRT‐PCR). Bioinformatics and circRNA/microRNA (miRNA) crosstalk analyses were applied to predict the potential functions of circRNAs. Finally, the miRNA expression was confirmed by miRNA sequencing. Results Compared with the control group, 6 members of circRNAs were up‐regulated, and 4 were down‐regulated in the ALD model. GO enrichment analyses revealed these circRNAs were predominantly enriched in the endoplasmic reticulum, arachidonic acid metabolism, and cytochrome P450 metabolism pathway. Among these circRNAs, the differential expression of 5 circRNAs was validated and consistent with qRT‐PCR, and only the up‐regulated mou_circ_1657 is included in the circBase. Further, the crosstalk analysis of circRNA–miRNA revealed 7 miRNAs were targeted by mou_circ_1657, of which miR‐19‐5b was the only miRNA that was down‐regulated in the ALD mice according to the miRNA sequencing data, suggesting it needs further attention in ALD. Conclusions This study demonstrates that a cluster of circRNAs is aberrantly expressed in the livers of ALD mice. mou_circ_1657/miR‐19‐5b may play a critical role in the development of ALD. Our study provides new insight into the future investigation and therapy on ALD.