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AIN‐93 Diet as an Alternative Model to Lieber–DeCarli Diet for Alcoholic Cardiomyopathy
Author(s) -
Cao Zhipeng,
Zhang Tianyi,
Xu Chengyang,
Jia Yuqing,
Wang Tianqi,
Zhu Baoli
Publication year - 2019
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/acer.14069
Subject(s) - alcoholic cardiomyopathy , cardiomyopathy , medicine , food science , chemistry , heart failure
Background The Lieber–DeCarli alcoholic liquid diet is a classical method for establishing animal models of alcoholic cardiomyopathy (ACM). No study has reported whether the AIN‐93 diet, which is widely used as a standard diet for both long‐term and short‐term studies with laboratory animals, could be used to construct the ACM animal model. The present study intended to investigate whether the AIN‐93 diet could be used to establish a mouse ACM model. Methods Twenty‐four C57BL/6 male mice were randomly divided into 4 equally sized groups. In ethanol (EtOH)‐fed groups, mice were fed a 4%‐EtOH (w/v, 28% of total calories) alcoholic liquid diet of Lieber–DeCarli or the AIN‐93 diet for chronic alcohol exposure for 180 days. In control‐fed groups, mice were fed with non‐EtOH liquid diets with the same calories as EtOH‐fed groups. Morphological observations of the hearts and molecular investigation of the brain natriuretic peptide (BNP) were carried out by echocardiography, hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining, real‐time quantitative polymerase chain reaction (qPCR), and enzyme‐linked immunosorbent assay. Results Echocardiography showed that mice fed with either the 4%‐EtOH Lieber–DeCarli diet or the 4%‐EtOH AIN‐93 diet had dilated ventricles and poor cardiac function. IHC staining of BNP, qPCR of BNP mRNA, and plasma concentration of BNP showed an up‐regulated expression in mice fed with both the 4%‐EtOH Lieber–DeCarli and 4%‐EtOH AIN‐93 diets. Less fatty liver was also observed in mice fed the AIN‐93 alcoholic diet than those fed the Lieber–DeCarli alcoholic diet. Conclusions The AIN‐93 alcoholic liquid diet can be used to establish ACM animal models, as with the conventional Lieber–DeCarli alcoholic liquid diet.