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Alcohol Exposure Causes Overexpression of Heart Development‐Related Genes by Affecting the Histone H3 Acetylation via BMP Signaling Pathway in Cardiomyoblast Cells
Author(s) -
Shi Jin,
Zhao Weian,
Pan Bo,
Zheng Min,
Si Lina,
Zhu Jing,
Liu Lingjuan,
Tian Jie
Publication year - 2017
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/acer.13273
Subject(s) - histone h3 , histone , hdac4 , histone deacetylase , acetylation , histone deacetylase 2 , histone deacetylase 5 , histone acetyltransferases , sap30 , histone methyltransferase , histone h4 , hdac11 , microbiology and biotechnology , biology , cancer research , biochemistry , gene
Background Abusive alcohol utilization of pregnant woman may cause congenital heart disease ( CHD ) of fetus, where alcohol ignites histone H3 hyperacetylation leading to abnormal development of heart morphogenesis and associated genes. Knowledge about the regularized upstream genes is little, but bone morphogenetic protein ( BMP ) signaling may actively and prominently take part in alteration in acetylation of histone H3. The supreme objective of this study was to unearth the involvement of BMP signaling pathway in alcohol‐driven hyperacetylation of histone H3 in cardiomyoblast cells. Methods Cardiomyoblast cells (H9c2 cells) were addicted with alcohol (100 mM) for 24 hours. Dorsomorphin (5 μ M) was used for the inhibition of BMP signaling pathway. We detected the phosphorylation activity of SMAD 1/5/8, mRNA expression, histone acetyltransferases ( HAT )/histone deacetylase ( HDAC ) activity, and acetylation of histone H3. Results Following alcohol exposure, phosphorylation of SMAD 1/5/8 and HAT activities was increased to a significant extent, while histone H3 acetylation and expression of heart development‐related genes were also increased. The said phenomenon influenced by alcohol was reverted upon dorsomorphin treatment to the cells without effecting HDAC activity. Conclusions The data clearly identified that BMP ‐mediated histone H3 acetylation of heart development‐related genes might be one of the possible cellular mechanisms to control alcohol‐induced expression of heart development‐related genes. Dorsomorphin, on the other hand, may modulate alcohol‐induced hyperacetylation of histone H3 through BMP targeting, which could be a potential way to block CHD .