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Similar Ethanol Drinking in Adolescent and Adult C 57 BL /6 J Mice After Chronic Ethanol Exposure and Withdrawal
Author(s) -
CarraraNascimento Priscila F.,
Lopez Marcelo F.,
Becker Howard C.,
Olive M. Foster,
Camarini Rosana
Publication year - 2013
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/acer.12056
Subject(s) - ethanol , alcohol , chemistry , inhalation , endocrinology , medicine , anesthesia , biochemistry
Background Increasing evidence shows that excessive alcohol consumption during adolescence increases vulnerability to alcohol use disorders in adulthood. The aim of this study was to examine differences between adolescent and adult C 57 BL /6J mice in drinking behavior and blood ethanol ( EtOH ) concentrations ( BEC s) after chronic EtOH exposure and withdrawal. Methods Male adolescent ( PND = 28 to 30) and adult ( PND = 70) C 57 BL /6 J mice were allowed to consume EtOH in a 2‐bottle choice paradigm (15% EtOH vs. water) for 3 weeks (Baseline drinking, Test 1, and Test 2), which were interspersed with 2 cycles ( C ycles I and II ) of chronic EtOH vapor or air inhalation (16 hours) and withdrawal (8 hours). BEC s were determined during both cycles. Results Chronic EtOH exposure led to increased EtOH intake during T est 1 and T est 2 in both adolescent and adult mice compared with air‐exposed controls, and no differences between age groups were observed. During C ycle I adult mice showed higher BEC s compared with adolescents. During C ycle II , BEC s were lower in adult mice as compared to C ycle I , and BEC s in adolescent mice did not change between the 2 cycles. Conclusions Chronic EtOH exposure followed by withdrawal periods increases EtOH consumption similarly in both adolescent and adult mice, despite differences in BEC s.