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The Effects of Rat Mesenchymal Stem Cells on Injury Progression In a Rat Model
Author(s) -
Singer Daniel D.,
Singer Adam J.,
Gordon Chris,
Brink Peter
Publication year - 2013
Publication title -
academic emergency medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.221
H-Index - 124
eISSN - 1553-2712
pISSN - 1069-6563
DOI - 10.1111/acem.12116
Subject(s) - medicine , mesenchymal stem cell , burn injury , saline , inflammation , wound healing , surgery , pathology , anesthesia
Objectives Burns are common injuries that can result in significant scarring, leading to poor function and disfigurement. Unlike mechanical injuries, burns often progress both in depth and in size over the first few days after injury, possibly due to inflammation and oxidative stress. A major gap in the field of burns is the lack of an effective therapy that reduces burn injury progression. Because stem cells have been shown to improve healing in several injury models, the authors hypothesized that species‐specific mesenchymal stem cells ( MSC s) would reduce injury progression in a rat comb‐burn model. Methods Using a brass comb preheated to 100°C, the authors created four rectangular burns, separated by three unburned interspaces on both sides of the backs of male Sprague‐Dawley rats. The interspaces represented the ischemic zones surrounding the central necrotic core. In an attempt to reduce burn injury progression, 20 rats were randomized to tail vein injections of 1 mL of rat‐specific MSCs, 10 6 cells/ mL ( n = 10), or normal saline ( n = 10), 60 minutes after injury. Results While the authors were unable to identify any quantum dot (Q‐dot)‐labeled MSC s in the injured skin, at 7 days the mean percentage of the unburned interspaces that became necrotic in the MSC group was significantly less than in the control group (80% vs. 100%, p < 0.0001). Conclusions Intravenous injection of rat MSC s reduced burn injury progression in a rat comb‐burn model.