Open AccessNAMPT reduction‐induced NAD + insufficiency contributes to the compromised oocyte quality from obese mice
Author(s) -
Wang Hengjie,
Zhu Shuai,
Wu Xinghan,
Liu Yuan,
Ge Juan,
Wang Qiang,
Gu Ling
Publication year - 2021
Publication title -
aging cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.103
H-Index - 140
eISSN - 1474-9726
pISSN - 1474-9718
DOI - 10.1111/acel.13496
Subject(s) - nad+ kinase , oocyte , nicotinamide adenine dinucleotide , biology , gene knockdown , endocrinology , medicine , nicotinamide , in vivo , microbiology and biotechnology , biochemistry , apoptosis , genetics , enzyme , embryo
Maternal obesity is associated with multiple adverse reproductive outcomes, whereas the underlying molecular mechanisms are still not fully understood. Here, we found the reduced nicotinamide phosphoribosyl transferase (NAMPT) expression and lowered nicotinamide adenine dinucleotide (NAD + ) content in oocytes from obese mice. Next, by performing morpholino knockdown assay and pharmacological inhibition, we revealed that NAMPT deficiency not only severely disrupts maturational progression and meiotic apparatus, but also induces the metabolic dysfunction in oocytes. Furthermore, overexpression analysis demonstrated that NAMPT insufficiency induced NAD + loss contributes to the compromised developmental potential of oocytes and early embryos from obese mice. Importantly, in vitro supplement and in vivo administration of nicotinic acid (NA) was able to ameliorate the obesity‐associated meiotic defects and oxidative stress in oocytes. Our results indicate a role of NAMPT in modulating oocyte meiosis and metabolism, and uncover the beneficial effects of NA treatment on oocyte quality from obese mice.