Open AccessAMPK‐mediated formation of stress granules is required for dietary restriction‐induced longevity in Caenorhabditis elegans
Author(s) -
Kuo ChenTing,
You GuanTing,
Jian YingJie,
Chen TingShin,
Siao YuChen,
Hsu AoLin,
Ching TsuiTing
Publication year - 2020
Publication title -
aging cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.103
H-Index - 140
eISSN - 1474-9726
pISSN - 1474-9718
DOI - 10.1111/acel.13157
Subject(s) - longevity , caenorhabditis elegans , biology , microbiology and biotechnology , ampk , starvation , stress granule , psychological repression , heat shock protein , heat shock , translation (biology) , genetics , messenger rna , endocrinology , gene , gene expression , protein kinase a , phosphorylation
Stress granules (SGs) are nonmembranous organelles that are dynamically assembled and disassembled in response to various stressors. Under stressed conditions, polyadenylated mRNAs and translation factors are sequestrated in SGs to promote global repression of protein synthesis. It has been previously demonstrated that SG formation enhances cell survival and stress resistance. However, the physiological role of SGs in organismal aging and longevity regulation remains unclear. In this study, we used TIAR‐1::GFP and GTBP‐1::GFP as markers to monitor the formation of SGs in Caenorhabditis elegans . We found that, in addition to acute heat stress, SG formation could also be triggered by dietary changes, such as starvation and dietary restriction (DR). We found that HSF‐1 is required for the SG formation in response to acute heat shock and starvation but not DR, whereas the AMPK‐eEF2K signaling is required for starvation and DR‐induced SG formation but not heat shock. Moreover, our data suggest that this AMPK‐eEF2K pathway‐mediated SG formation is required for lifespan extension by DR, but dispensable for the longevity by reduced insulin/IGF‐1 signaling. Collectively, our findings unveil a novel role of SG formation in DR‐induced longevity.