Open AccessLysophosphatidic acid receptor LPA 3 prevents oxidative stress and cellular senescence in Hutchinson–Gilford progeria syndrome
Author(s) -
Chen WeiMin,
Chiang JuiChung,
Lin YuehChien,
Lin YuNung,
Chuang PeiYun,
Chang YaChi,
Chen ChienChin,
Wu KaoYi,
Hsieh JungChien,
Chen ShihKuo,
Huang WeiPang,
Chen Benjamin P. C.,
Lee Hsinyu
Publication year - 2020
Publication title -
aging cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.103
H-Index - 140
eISSN - 1474-9726
pISSN - 1474-9718
DOI - 10.1111/acel.13064
Subject(s) - lysophosphatidic acid , progeria , biology , microbiology and biotechnology , premature aging , oxidative stress , senescence , zebrafish , reactive oxygen species , receptor , biochemistry , genetics , gene
Hutchinson–Gilford progeria syndrome (HGPS) is a rare laminopathy that produces a mutant form of prelamin A, known as Progerin, resulting in premature aging. HGPS cells show morphological abnormalities of the nuclear membrane, reduced cell proliferation rates, accumulation of reactive oxygen species (ROS), and expression of senescence markers. Lysophosphatidic acid (LPA) is a growth factor‐like lipid mediator that regulates various physiological functions via activating multiple LPA G protein‐coupled receptors. Here, we study the roles of LPA and LPA receptors in premature aging. We report that the protein level of LPA 3 was highly downregulated through internalization and the lysosomal degradation pathway in Progerin‐transfected HEK293 cells. By treating Progerin HEK293 cells with an LPA 3 agonist (OMPT, 1‐Oleoyl‐2‐O‐methyl‐rac‐glycerophosphothionate) and performing shRNA knockdown of the Lpa3r transcript in these cells, we showed that LPA 3 activation increased expression levels of antioxidant enzymes, consequently inhibiting ROS accumulation and ameliorating cell senescence. LPA 3 was shown to be downregulated in HGPS patient fibroblasts through the lysosomal pathway, and it was shown to be crucial for ameliorating ROS accumulation and cell senescence in fibroblasts. Moreover, in a zebrafish model, LPA 3 deficiency was sufficient to cause premature aging phenotypes in multiple organs, as well as a shorter lifespan. Taken together, these findings identify the decline of LPA 3 as a key contributor to the premature aging phenotypes of HGPS cells and zebrafish.