Open AccessLong‐term repopulation of aged bone marrow stem cells using young Sca‐1 cells promotes aged heart rejuvenation
Author(s) -
Li Jiao,
Li ShuHong,
Dong Jun,
Alibhai Faisal J.,
Zhang Chongyu,
Shao ZhengBo,
Song HuiFang,
He Sheng,
Yin WenJuan,
Wu Jun,
Weisel Richard D.,
Liu ShiMing,
Li RenKe
Publication year - 2019
Publication title -
aging cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.103
H-Index - 140
eISSN - 1474-9726
pISSN - 1474-9718
DOI - 10.1111/acel.13026
Subject(s) - biology , stem cell , homing (biology) , telomerase , bone marrow , cxcr4 , bmi1 , cd34 , cancer research , chimera (genetics) , microbiology and biotechnology , immunology , chemokine , inflammation , gene , genetics , ecology
Reduced quantity and quality of stem cells in aged individuals hinders cardiac repair and regeneration after injury. We used young bone marrow (BM) stem cell antigen 1 (Sca‐1) cells to reconstitute aged BM and rejuvenate the aged heart, and examined the underlying molecular mechanisms. BM Sca‐1 + or Sca‐1 − cells from young (2–3 months) or aged (18–19 months) GFP transgenic mice were transplanted into lethally irradiated aged mice to generate 4 groups of chimeras: young Sca‐1 + , young Sca‐1 − , old Sca‐1 + , and old Sca‐1 − . Four months later, expression of rejuvenation‐related genes (Bmi1, Cbx8, PNUTS, Sirt1, Sirt2, Sirt6) and proteins (CDK2, CDK4) was increased along with telomerase activity and telomerase‐related protein (DNA‐PKcs, TRF‐2) expression, whereas expression of senescence‐related genes (p16 INK4a , P19 ARF , p27 Kip1 ) and proteins (p16 INK4a , p27 Kip1 ) was decreased in Sca‐1 + chimeric hearts, especially in the young group. Host cardiac endothelial cells (GFP − CD31 + ) but not cardiomyocytes were the primary cell type rejuvenated by young Sca‐1 + cells as shown by improved proliferation, migration, and tubular formation abilities . C‐X‐C chemokine CXCL12 was the factor most highly expressed in homed donor BM (GFP + ) cells isolated from young Sca‐1 + chimeric hearts. Protein expression of Cxcr4, phospho‐Akt, and phospho‐FoxO3a in endothelial cells derived from the aged chimeric heart was increased, especially in the young Sca‐1 + group. Reconstitution of aged BM with young Sca‐1 + cells resulted in effective homing of functional stem cells in the aged heart. These young, regenerative stem cells promoted aged heart rejuvenation through activation of the Cxcl12/Cxcr4 pathway of cardiac endothelial cells.