Open AccessExpression of p16 INK 4a is a biomarker of chondrocyte aging but does not cause osteoarthritis
Author(s) -
Diekman Brian O.,
Sessions Garrett A.,
Collins John A.,
Knecht Anne K.,
Strum Susan L.,
Mitin Natalia K.,
Carlson Cathy S.,
Loeser Richard F.,
Sharpless Norman E.
Publication year - 2018
Publication title -
aging cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.103
H-Index - 140
eISSN - 1474-9726
pISSN - 1474-9718
DOI - 10.1111/acel.12771
Subject(s) - senescence , chondrocyte , biology , microbiology and biotechnology , cartilage , osteoarthritis , cyclin dependent kinase , cancer research , cdk inhibitor , cell cycle , cell , pathology , medicine , genetics , anatomy , alternative medicine
Summary Cellular senescence drives a functional decline of numerous tissues with aging by limiting regenerative proliferation and/or by producing pro‐inflammatory molecules known as the senescence‐associated secretory phenotype ( SASP ). The senescence biomarker p16 INK4a is a potent inhibitor of the cell cycle but is not essential for SASP production. Thus, it is unclear whether p16 INK4a identifies senescence in hyporeplicative cells such as articular chondrocytes and whether p16 INK4a contributes to pathologic characteristics of cartilage aging. To address these questions, we examined the role of p16 INK4a in murine and human models of chondrocyte aging. We observed that p16 INK4a mRNA expression was significantly upregulated with chronological aging in murine cartilage (~50‐fold from 4 to 18 months of age) and in primary human chondrocytes from 57 cadaveric donors ( r 2 = .27, p < .0001). Human chondrocytes exhibited substantial replicative potential in vitro that depended on the activity of cyclin‐dependent kinases 4 or 6 ( CDK 4/6), and proliferation was reduced in cells from older donors with increased p16 INK4a expression. Moreover, increased chondrocyte p16 INK4a expression correlated with several SASP transcripts. Despite the relationship between p16 INK4a expression and these features of senescence, somatic inactivation of p16 INK4a in chondrocytes of adult mice did not mitigate SASP expression and did not alter the rate of osteoarthritis ( OA ) with physiological aging or after destabilization of the medial meniscus. These results establish that p16 INK4a expression is a biomarker of dysfunctional chondrocytes, but that the effects of chondrocyte senescence on OA are more likely driven by production of SASP molecules than by loss of chondrocyte replicative function.