Open AccessRobust, universal biomarker assay to detect senescent cells in biological specimens
Author(s) -
Evangelou Konstantinos,
Lougiakis Nikolaos,
Rizou Sophia V.,
Kotsinas Athanassios,
Kletsas Dimitris,
MuñozEspín Daniel,
Kastrinakis Nikolaos G,
Pouli Nicole,
Marakos Panagiotis,
Townsend Paul,
Serrano Manuel,
Bartek Jiri,
Gorgoulis Vassilis G.
Publication year - 2017
Publication title -
aging cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.103
H-Index - 140
eISSN - 1474-9726
pISSN - 1474-9718
DOI - 10.1111/acel.12545
Subject(s) - biology , lipofuscin , biomarker , senescence , computational biology , biomedicine , cellular senescence , microbiology and biotechnology , cellular aging , bioinformatics , telomere , biochemistry , dna , gene , phenotype
Summary Cellular senescence contributes to organismal development, aging, and diverse pathologies, yet available assays to detect senescent cells remain unsatisfactory. Here, we designed and synthesized a lipophilic, biotin‐linked Sudan Black B ( SBB ) analogue suitable for sensitive and specific, antibody‐enhanced detection of lipofuscin‐containing senescent cells in any biological material. This new hybrid histo‐/immunochemical method is easy to perform, reliable, and universally applicable to assess senescence in biomedicine, from cancer research to gerontology.