Open AccessFibroblasts from long‐lived species of mammals and birds show delayed, but prolonged, phosphorylation of ERK
Author(s) -
Elbourkadi Najoua,
Austad Steven N.,
Miller Richard A.
Publication year - 2014
Publication title -
aging cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.103
H-Index - 140
eISSN - 1474-9726
pISSN - 1474-9718
DOI - 10.1111/acel.12172
Subject(s) - phosphorylation , mapk/erk pathway , biology , cadmium , hydrogen peroxide , kinase , microbiology and biotechnology , mutant , biochemistry , chemistry , gene , organic chemistry
Summary Fibroblasts from long‐lived mutant mice show diminished phosphorylation of the stress‐activated protein kinases ERK 1/2 after exposure to peroxide, cadmium, or paraquat. We have now evaluated the kinetics of ERK phosphorylation in fibroblasts from long‐lived and short‐lived species of mammals and birds in response to stress by cadmium or hydrogen peroxide. Fibroblasts from the shorter‐lived species of rodents and birds showed rapid induction of ERK phosphorylation, with a decline to basal level within 60 min. In contrast, cells from longer‐lived species showed slower and more prolonged activation of ERK phosphorylation. These results suggest that fibroblasts from long‐lived species may be less susceptible to the early phases of damage from cadmium or peroxide and suggest that altered kinetics of ERK activity may contribute to their stress resistance properties.