Open AccessMitochondrial energetics is impaired in vivo in aged skeletal muscle
Author(s) -
Gouspillou Gilles,
BourdelMarchasson Isabelle,
Rouland Richard,
Calmettes Guillaume,
Biran Marc,
DeschodtArsac Véronique,
Miraux Sylvain,
Thiaudiere Eric,
Pasdois Philippe,
Detaille Dominique,
Franconi JeanMichel,
Babot Marion,
Trézéguet Véronique,
Arsac Laurent,
Diolez Philippe
Publication year - 2014
Publication title -
aging cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.103
H-Index - 140
eISSN - 1474-9726
pISSN - 1474-9718
DOI - 10.1111/acel.12147
Subject(s) - bioenergetics , skeletal muscle , sarcopenia , mitochondrion , oxidative phosphorylation , in vivo , biology , medicine , endocrinology , microbiology and biotechnology , biochemistry , genetics
Summary With aging, most skeletal muscles undergo a progressive loss of mass and strength, a process termed sarcopenia. Aging‐related defects in mitochondrial energetics have been proposed to be causally involved in sarcopenia. However, changes in muscle mitochondrial oxidative phosphorylation with aging remain a highly controversial issue, creating a pressing need for integrative approaches to determine whether mitochondrial bioenergetics are impaired in aged skeletal muscle. To address this issue, mitochondrial bioenergetics was first investigated in vivo in the gastrocnemius muscle of adult (6 months) and aged (21 months) male W istar rats by combining a modular control analysis approach with 31 P magnetic resonance spectroscopy measurements of energetic metabolites. Using this innovative approach, we revealed that the in vivo responsiveness (‘elasticity’) of mitochondrial oxidative phosphorylation to contraction‐induced increase in ATP demand is significantly reduced in aged skeletal muscle, a reduction especially pronounced under low contractile activities. In line with this in vivo aging‐related defect in mitochondrial energetics, we found that the mitochondrial affinity for ADP is significantly decreased in mitochondria isolated from aged skeletal muscle. Collectively, the results of this study demonstrate that mitochondrial bioenergetics are effectively altered in vivo in aged skeletal muscle and provide a novel cellular basis for this phenomenon.