Open AccessAging results in copper accumulations in glial fibrillary acidic protein‐positive cells in the subventricular zone
Author(s) -
Pushkar Yulia,
Robison Gregory,
Sullivan Brendan,
Fu Sherleen X.,
Kohne Meghan,
Jiang Wendy,
Rohr Sven,
Lai Barry,
Marcus Matthew A.,
Zakharova Taisiya,
Zheng Wei
Publication year - 2013
Publication title -
aging cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.103
H-Index - 140
eISSN - 1474-9726
pISSN - 1474-9718
DOI - 10.1111/acel.12112
Subject(s) - subventricular zone , glial fibrillary acidic protein , neurogenesis , biology , copper , immunohistochemistry , microbiology and biotechnology , neural stem cell , biophysics , chemistry , immunology , stem cell , organic chemistry
Summary Analysis of rodent brains with X ‐ray fluorescence ( XRF ) microscopy combined with immunohistochemistry allowed us to demonstrate that local C u concentrations are thousands of times higher in the glia of the subventricular zone ( SVZ ) than in other cells. Using XRF microscopy with subcellular resolution and intracellular X ‐ray absorption spectroscopy we determined the copper (I) oxidation state and the sulfur ligand environment. C u K ‐edge X‐ray absorption near edge spectroscopy is consistent with C u being bound as a multimetallic C u‐ S cluster similar to one present in C u‐metallothionein. Analysis of age‐related changes show that C u content in astrocytes of the SVZ increases fourfold from 3 weeks to 9 months, while C u concentration in other brain areas remain essentially constant. This increase in C u correlates with a decrease in adult neurogenesis assessed using the K i67 marker (both, however, can be age‐related effects). We demonstrate that the C u distribution and age‐related concentration changes in the brain are highly cell specific.