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Kinetics of leukocyte CD11b and CD64 expression in severe sepsis and non‐infectious critical care patients
Author(s) -
Jämsä J.,
Huotari V.,
Savolainen E.R.,
Syrjälä H.,
Alakokko T.
Publication year - 2015
Publication title -
acta anaesthesiologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.738
H-Index - 107
eISSN - 1399-6576
pISSN - 0001-5172
DOI - 10.1111/aas.12515
Subject(s) - medicine , sepsis , monocyte , intensive care unit , cd64 , septic shock , gastroenterology , integrin alpha m , systemic inflammatory response syndrome , immunology , systemic inflammation , inflammation , flow cytometry
Background Leukocyte surface molecules may improve sepsis diagnostics. Our aim was to study whether monocyte and neutrophil CD 11b and CD 64 expression differs between patients with severe sepsis (including septic shock) and intensive care unit ( ICU ) controls, and also to investigate the expression kinetics in patient groups. Methods Monocyte and neutrophil CD 11b and CD 64 expression was analyzed in 27 patients with severe sepsis, 7 off‐pump coronary artery bypass ( OPCAB ) patients, and 8 ICU patients without systemic inflammation in the beginning of the treatment using quantitative flow cytometry. Blood samples were collected within 48 h of the beginning of severe sepsis, at admission to the ICU for non‐systemic inflammatory response syndrome patients, and on the day of surgery before the skin incision for OPCAB patients, and on 2 consecutive days for all patients. Ten healthy individuals served as controls. Results Monocyte and neutrophil CD 11b and neutrophil CD 64 expression was higher in severe sepsis patients compared with the other groups ( P < 0.05). In severe sepsis, the expression decreased over time ( P < 0.05). In OPCAB patients, the monocyte and neutrophil CD 64 expression increased after surgery ( P < 0.05). Neutrophil CD 64 expression had the highest and statistically significant area under curves ( AUC ) values for identification of severe sepsis during 3 consecutive days, the highest AUC being 0.990 on D 0. Conclusion Neutrophil CD 64 as well as neutrophil and monocyte CD 11b expressions were highest in severe sepsis compared with non‐infectious conditions, and thus analyses of their expression may be promising approach for sepsis diagnosis in ICU population.