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Sevoflurane causes neuronal apoptosis and adaptability changes of neonatal rats
Author(s) -
ZHENG S. Q.,
AN L. X.,
CHENG X.,
WANG Y. J.
Publication year - 2013
Publication title -
acta anaesthesiologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.738
H-Index - 107
eISSN - 1399-6576
pISSN - 0001-5172
DOI - 10.1111/aas.12163
Subject(s) - sevoflurane , morris water navigation task , medicine , hippocampus , anesthesia , open field , apoptosis , neurodegeneration , endocrinology , biology , biochemistry , disease
Background Neonatal exposure to sevoflurane can induce neurodegeneration and learning deficits in developing brain. We hypothesised that with the increase in the concentration and duration of sevoflurane, neurodegeneration of neonatal rats aggravates and causes behaviour changes as the rats grow. Methods Twenty‐one post‐natal day ( P )7 Wistar rats were randomly divided into seven groups. Blood analysis was performed after anaesthesia. According to the results, 120 P7 Wistar rats were randomly divided into five groups: Con sham anaesthesia; S evo 1%‐2 h: exposed to 1% sevoflurane for 2 h; S evo 1%‐4 h, S evo 2%‐2 h and Sevo 2%‐4 h. Caspase‐3 positive cells in brain were detected by immunohistochemistry at 6 h after the end of anaesthesia. The cleaved poly( ADP ‐ribose) polymerase (c‐ PARP ‐1) in cortex and hippocampus was detected by Western blot analysis. Behavioural tests such as M orris water maze and Open‐field Test were performed when the rats were 5‐week old, 8‐week old, and 14‐week old. Results Three per cent sevoflurane induced carbon dioxide accumulation. The level of c‐ PARP ‐1 in hippocampus area was significantly increased in Group 2%‐4h. The number of caspase‐3 positive cells in Group S evo 1%‐2h, Group S evo 2%‐2h and Group S evo 2%‐4h was greater than that in Group C on. Rats exposed to sevoflurane had longer travel distance and time in open field when they were 5 weeks old. Animals from different groups had similar performance in M orris water maze. Conclusion Exposure to 2% sevoflurane causes neuronal apoptosis of neonatal rats, and long‐time exposure aggravates that. The adaptability in new environment is transiently decreased when the anaesthesia rats are 5 weeks old.