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A loop‐mediated isothermal amplification assay for the detection of Dahlia mosaic caulimovirus in Dahlia ( Dahlia variabilis )
Author(s) -
Iftikhar Romana,
Moyo Lindani,
Pappu Hanu R.
Publication year - 2020
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/aab.12574
Subject(s) - dahlia , loop mediated isothermal amplification , biology , virology , microbiology and biotechnology , dna , horticulture , genetics
Dahlia mosaic virus (DMV), an aphid‐transmitted caulimovirus in the family Caulimoviridae , is an important pathogen of dahlia ( Dahlia variabilis ). Development of rapid, and sensitive diagnostic tools is essential in surveillance and management of DMV. A loop‐mediated isothermal amplification (LAMP) assay was developed for DMV detection. The LAMP assay targets the reverse transcriptase region of the DMV genome and is optimal at a temperature of 60°C and run time of 60 min, and amplification was detected through fluorescence detection and by agarose gel electrophoresis. The assay detected DMV in DNA concentration as low as 10 −3 ng. To the best of our knowledge, this is the first report of a LAMP assay for the detection of DMV, a plant DNA virus. This assay will be useful in rapid and sensitive detection of DMV and in reducing the virus incidence through production of virus‐free planting material.