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Multilocus sequence analysis of phytoplasma strains of 16SrII group in Iran and their comparison with related strains
Author(s) -
Siampour Majid,
Izadpanah Keramatollah,
Martini Marta,
Salehi Mohammad
Publication year - 2019
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/aab.12513
Subject(s) - biology , phytoplasma , phylogenetic tree , genetic diversity , lineage (genetic) , 16s ribosomal rna , phylogenetics , genetics , ribosomal rna , evolutionary biology , gene , polymerase chain reaction , restriction fragment length polymorphism , population , demography , sociology
Phytoplasmas of the group 16SrII (peanut witches'‐broom group) are among the most important phytoplasmas identified in Iran. These phytoplasmas are so diverse that they have been classified within 23 subgroups, among which phytoplasmas of subgroups 16SrII‐B, ‐C and ‐D have been recognised in Iran. In this study, we used multilocus sequence analysis as a tool to find the extent of genetic diversity and phylogeny of representative phytoplasmas of 16SrII in Iran in comparison to reference phytoplasma strains characterised elsewhere. The genes used were 16S rRNA, secY , rplV ‐ rpsC , imp and a hypothetical protein ( inmp ). Analysis of this study showed that phytoplasmas of 16SrII could be resolved into at least three main phylogenetic lineages. One lineage comprised phytoplasmas of the subgroups 16SrII‐A and II‐D, another included strains of subgroups 16SrII‐B and II‐C and the third lineage comprised phytoplasmas belonging to 16SrII‐E. The significance of host adaptation and geographical distribution in relation to the genetic diversity of these phytoplasmas is discussed. Among five different genetic loci used in this study, imp gene displayed the highest genetic diversity, hence considered as the most powerful genetic tool for differentiation of closely related phytoplasmas.