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Phosphate solubilisation and plant growth promoting potential by stress tolerant Bacillus sp. isolated from rhizosphere of apple orchards in trans Himalayan region of Himachal Pradesh
Author(s) -
Mehta P.,
Walia A.,
Chauhan A.,
Kulshrestha S.,
Shirkot C. K.
Publication year - 2013
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/aab.12077
Subject(s) - rhizosphere , bacillus subtilis , siderophore , gluconic acid , biology , rhizobacteria , phosphate , citric acid , food science , salt (chemistry) , organic acid , acetic acid , horticulture , botany , biochemistry , bacteria , chemistry , genetics , gene
Phosphate‐solubilising ability and co‐production of plant growth promoting traits of stress tolerant Bacillus subtilis CB 8 A isolated from apple rhizosphere was tested under in vitro conditions against a wide range of temperature (30–45°C), pH (7–9) and salt (0–5%) stresses. Under the extremes of temperature (45°C), pH ‐9 and salt concentration (5%), production of soluble phosphate, indole acetic acid, siderophore and antifungal activity against Dematophora necatrix were reduced by 71.09%, 75.29%, 90.3% and 88.47%, respectively. Per cent decrease in P‐solubilisation at extreme temperature (45°C) and normal pH (7) without salt concentration was 36.23%; at extreme pH (9) and normal temperature (37°C) without salt concentration was 23.45% and at extreme salt concentration (5%), optimum temperature (37°C) and pH (7) was 36.7%. P‐solubilisation by CB 8 A was inversely correlated with pH ( r = −0.78) and positively correlated with siderophore production ( r = 0.81), indole acetic acid ( r = 0.58) and antifungal activity ( r = 0.63). Gluconic acid (1.43%) and citric acid (0.67%) were detected as major organic acids. P‐solubilisation and nitrogen fixing abilities of B. subtilis CB 8 A were confirmed by amplification of gdh and nif H genes. The ability of CB8A showing plant growth promoting rhizobacteria ( PGPR ) traits at a wide range of temperature, pH and varying salt concentration can be exploited for developing multifunctional biofertiliser in apple orchards.