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Focused ultrasound extraction (FUSE) for the rapid extraction of DNA from tissue matrices
Author(s) -
Holmes Hal R.,
Haywood Morgan,
Hutchison Ruby,
Zhang Qian,
Edsall Connor,
Hall Timothy L.,
Baisch David,
Holliday Jason,
Vlaisavljevich Eli
Publication year - 2020
Publication title -
methods in ecology and evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.425
H-Index - 105
ISSN - 2041-210X
DOI - 10.1111/2041-210x.13505
Subject(s) - extraction (chemistry) , dna extraction , fuse (electrical) , dna , salmo , computer science , computational biology , biology , chromatography , fishery , chemistry , polymerase chain reaction , engineering , genetics , gene , fish <actinopterygii> , electrical engineering
Rapid DNA extraction is a critical barrier for routine and fieldable genetics tests for applications in conservation, such as illegal trafficking and fraudulent mislabelling. Here, we develop a non‐thermal focused ultrasound extraction (FUSE) technique that creates a dense cloud of high‐pressure acoustic cavitation bubbles to disintegrate targeted tissues into an acellular debris, resulting in the rapid release of entrapped DNA. In this work, we demonstrate the proof‐of‐concept of the FUSE technique by obtaining species identifiable sequences and shotgun sequencing reads from DNA extracted from Atlantic salmon Salmo salar tissues. Having mitigated the key risks for this technique, we hypothesize future developments with this technology can be applied to accelerate and simplify DNA extraction from exceedingly difficult samples with complex tissue matrices (i.e. fibrous tissue and timber samples) in both laboratory and field settings.