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Use of dd PCR in experimental evolution studies
Author(s) -
Koch Hanna,
Jeschke Alina,
Becks Lutz
Publication year - 2016
Publication title -
methods in ecology and evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.425
H-Index - 105
ISSN - 2041-210X
DOI - 10.1111/2041-210x.12467
Subject(s) - genotyping , biology , multiplex , experimental evolution , computational biology , throughput , evolutionary biology , genotype , genetic diversity , adaptation (eye) , range (aeronautics) , genetics , computer science , gene , population , telecommunications , demography , materials science , composite material , neuroscience , sociology , wireless
Summary Experimental evolution is an important research framework for evolutionary biologists as it allows direct testing of fundamental theories about adaptation and diversity, which often requires the tracking of genotypes or alleles over time. This however requires tools such as genetic markers, distinguishable morphological characters or genotyping, which can be time and labour intensive, and especially if high‐throughput processing is necessary. Here, we present a novel approach of combining multiplex dd PCR with experimental evolution for tracking genotype frequencies in different experimental populations over time. We found this method to be both precise and accurate for detecting and quantifying targets of interest, especially as compared to traditional PCR methods. The use of multiplex dd PCR to follow frequencies can be used for a wide range of experimental evolution applications.