Open AccessProtein tyrosine phosphatase PTPL1 suppresses lung cancer through Src/ ERK / YAP1 signaling
Author(s) -
Wang Jing,
Li Shuanghui,
Zhang Xiujuan,
Zhu Ning,
Yiminniyaze Ruzetuoheti,
Dong Liang,
Li Chengwei,
Gulinuer Wumaier,
Xia Jingwen,
Li Jing,
Zhou Daibing,
Liu Xinning,
Zhang Youzhi,
Zhang Yuanyuan,
Li Shengqing
Publication year - 2022
Publication title -
thoracic cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 28
eISSN - 1759-7714
pISSN - 1759-7706
DOI - 10.1111/1759-7714.14657
Subject(s) - yap1 , cancer research , mapk/erk pathway , proto oncogene tyrosine protein kinase src , gene knockdown , cell growth , lung cancer , microbiology and biotechnology , tyrosine kinase , protein tyrosine phosphatase , medicine , signal transduction , biology , cell culture , pathology , biochemistry , genetics , gene , transcription factor
Background To reveal the function of protein tyrosine phosphatase‐L1 (PTPL1) in lung adenocarcinoma. Methods Lung cancer cell lines were transfected with short hairpin RNA against PTPL1 (shPTPL1 group) or negative control (shmock group). Quantitative real‐time polymerase chain reaction (qRT‐PCR) and western blotting were used to verify the transfection efficacy. Cell proliferation was analyzed by ethynyldeoxyuridine (EdU), Cell counting kit 8 (CCK8), and colony formation assay after PTPL1 or PTPL1 and yes‐associated protein (YAP1) knockdown. The effect of PTPL1 on tumor growth was examined in a xenograft lung cancer model. Results PTPL1 was downregulated in various types of lung cancer cell lines. The EdU, CCK8, colony formation assays and investigation using a xenograft lung cancer model indicated that PTPL1 knockdown increased the proliferation of lung cancer cells. Mechanistically, PTPL1 knockdown induced the activation of the Proto‐oncogene tyrosine‐protein kinase SRC (Src)/Extracellular regulated MAP kinase (ERK) pathway and thereby promoted yes‐associated protein (YAP1) nuclear translocation and activation. Conclusions In our study, PTPL1 played a crucial suppressive role in the pathogenesis of lung cancer potentially through counteracting the Src/ERK/YAP1 pathway.