Open AccessCloning short DNA into plasmids by one‐step PCR
Author(s) -
Tao ChengCheng,
Yang Ying,
Li Fang,
Qiao Ling,
Wu Yue,
Sun XiaoDong,
Zhang YuanYuan,
Li ChangLong
Publication year - 2020
Publication title -
thoracic cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 28
eISSN - 1759-7714
pISSN - 1759-7706
DOI - 10.1111/1759-7714.13660
Subject(s) - plasmid , microbiology and biotechnology , transformation (genetics) , clone (java method) , cloning (programming) , plasmid preparation , polymerase chain reaction , ligation , t dna binary system , molecular cloning , transfection , dna , cloning vector , gene , biology , recombinant dna , pbr322 , genetics , gene expression , vector (molecular biology) , computer science , programming language
Background Plasmid construction of small fragments of interest (such as insertion of small fragment marker genes, expression of shRNA, siRNA, etc) is the basis of many biomolecular experiments. Here, we describe a method to clone short DNA into vectors by polymerase chain reaction (PCR), named one‐step PCR cloning. Our method uses PCR to amplify the entire circular plasmid. The PCR was performed by the primers containing the gene of short DNA with overlapping sequences between 10–15 bp. The PCR products were then transformed into E. coli and cyclized by homologous recombination in vivo. Methods The pEGFP‐N1‐HA plasmid was constructed by one‐step PCR and transformation. Cells were transfected with pEGFP‐N1‐HA and pEGFP‐N1 plasmid using TurboFect transfection reagent. Protein expression was detected by western blotting and the HA‐GFP fusion protein was detected by confocal microscopy. Results The pEGFP‐N1‐HA plasmid was successfully constructed and HA expression in cells. Conclusions Free from the limitations of restriction enzyme sites and omitting the ligation process, our method offers a flexible and economical option of plasmid construction. Key points Significant findings of the study A method to clone short DNA into plasmids was found. What this study adds Our study provides a flexible and economical option to clone short DNA into plasmids.