Open AccessUpregulation of E‐cadherin in bronchoalveolar lavage fluid‐derived exosomes in patients with lung cancer
Author(s) -
Zhang Ying,
Liu Ziyu,
Li Shanyu,
Wang Manning,
Dai Dayou,
Jing Hongyu,
Liu Lingyun
Publication year - 2020
Publication title -
thoracic cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 28
eISSN - 1759-7714
pISSN - 1759-7706
DOI - 10.1111/1759-7714.13220
Subject(s) - bronchoalveolar lavage , microvesicles , lung cancer , medicine , metastasis , lung , cancer , downregulation and upregulation , cancer research , pathology , immunology , microrna , biology , biochemistry , gene
Background Lung cancer features extremely high rates of morbidity and mortality. Bronchoalveolar lavage fluid (BALF), obtained by bronchoscopy and bronchoalveolar perfusion, can provide information on the cellular components of the lung microenvironment to assist with diagnosis and treatment of lung cancer. Methods BALF was performed using a flexible bronchofiberscope. Exosomes were collected by ultracentrifugation. ELISA detected the amount of E‐cadherin. Transmission electron microscopic, ELISA and WB were conducted to identify the existence of the exosomes. Transwell and Wound healing assays were used to detect the ability of migration and invasion. Results We identified the existence of exosomes in BALF. Furthermore, we observed larger amounts of E‐cadherin in the BALF obtained from patients with lung cancer than in the control obtained from the healthy side of pneumonia. Exosomes from lung cancer groups promoted the migration and invasion of A549 cancer cells. Conclusion The exosomes from lung cancer BALF promoted the migration and invasion of A549 cancer cells by carrying E‐cadherin. E‐cadherin on the surface of exosomes may act through a VE‐cadherin dependent mechanism and induce lung cancer metastasis.