Open AccessLong non‐coding RNA linc01433 promotes migration and invasion in non‐small cell lung cancer
Author(s) -
Qian Banglun,
Wang Xiang,
Mao Chao,
Jiang Yiqun,
Shi Ying,
Chen Ling,
Liu Shuang,
Wang Bin,
Pan Shu,
Tao Yongguang,
Shi Hongcan
Publication year - 2018
Publication title -
thoracic cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 28
eISSN - 1759-7714
pISSN - 1759-7706
DOI - 10.1111/1759-7714.12623
Subject(s) - medicine , lung cancer , long non coding rna , cancer research , rna , coding (social sciences) , computational biology , oncology , gene , genetics , biology , statistics , mathematics
Background For many years, lung cancer has been the most common and deadly cancer worldwide. Early diagnosis of non‐small cell lung cancer (NSCLC) in particular is very difficult because the symptoms are often ignored. The five‐year survival rate is very low despite great improvements to therapy. Thus, there is an urgent need to identify prognostic biomarkers and target molecules for the clinical diagnosis and individualized treatment of NSCLC. Methods We performed quantitative real‐time PCR to determine the expression levels of the long non‐coding RNA (lncRNA) linc01433 in NSCLC and normal matched lung tissue. Subsequently, we established cell lines with overexpression or knockdown of linc01433 to evaluate the effects on proliferation and metastasis in vitro. Epithelial‐to‐mesenchymal transition was examined using Western blot. Results Linc01433 was significantly overexpressed in NSCLC tissues compared to normal lung tissues. In addition, linc01433 levels were associated with smoking history. Linc01433 overexpression in lung cancer cells increased proliferation, migration, and invasion abilities, as well as epithelial‐to‐mesenchymal transition. Conclusions Linc01433 is a cancer‐related lncRNA that may have an oncogene‐like effect in NSCLC.