Open AccessEffects of FSTL 1 on the proliferation and motility of breast cancer cells and vascular endothelial cells
Author(s) -
Yang Yang,
Mu Tianhao,
Li Te,
Xie Songbo,
Zhou Jun,
Liu Min,
Li Dengwen
Publication year - 2017
Publication title -
thoracic cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 28
eISSN - 1759-7714
pISSN - 1759-7706
DOI - 10.1111/1759-7714.12491
Subject(s) - angiogenesis , cancer cell , microbiology and biotechnology , motility , medicine , endothelial stem cell , cancer research , biology , cancer , in vitro , biochemistry
Background Treatments that prevent the motility of breast cancer cells and inhibit formation of new capillary vessels are urgently needed. FSTL 1 is a secreted protein that has been implicated in maintaining the normal physiological function of the cardiovascular system, in addition to a variety of other biological functions. We investigated the role of FSTL1 in the proliferation and migration of breast cancer and vascular endothelial cells. Methods Human umbilical vein endothelial cells and human breast cancer BT‐549 cells were used to test the effects of FSTL1 and the N‐terminal domain of FSTL1. Immunofluorescence microscopy and 3‐(4, 5‐dimethylthiazolyl‐2)‐2,5‐diphenyltetrazolium bromide, transwell invasion, and wound healing assays were conducted. Results Different doses of the N ‐terminal fragment of FSTL 1 ( FSTL ‐ N ) have variable effects on the migration of these cells. However, FSTL 1 does not significantly affect tube formation in vitro from vascular endothelial cells. FSTL 1‐ FL and FSTL 1‐ N have modest effects on the invasion of breast cancer and vascular endothelial cells. Interestingly, FSTL 1‐ FL , but not FSTL ‐ N , modulates vascular endothelial cell polarization. Conclusion FSTL 1 modestly affects the proliferation of breast cancer cells and vascular endothelial cells. Our findings improve our understanding of the functions of FSTL 1 in breast cancer development and angiogenesis.