Open AccessAssociation between polymorphisms in micro RNA target sites and survival in early‐stage non‐small cell lung cancer
Author(s) -
Yoo Seung Soo,
Hong Mi Jeong,
Lee Jang Hyuck,
Choi Jin Eun,
Lee Shin Yup,
Lee Jaehee,
Cha Seung Ick,
Kim Chang Ho,
Seok Yangki,
Lee Eungbae,
Cho Sukki,
Jheon Sanghoon,
Park Jae Yong
Publication year - 2017
Publication title -
thoracic cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 28
eISSN - 1759-7714
pISSN - 1759-7706
DOI - 10.1111/1759-7714.12478
Subject(s) - hazard ratio , medicine , lung cancer , stage (stratigraphy) , confidence interval , oncology , proportional hazards model , genotype , rna , gene , gastroenterology , genetics , biology , paleontology
A high‐throughput mapping method of RNA – RNA interactions by crosslinking, ligation, and sequencing of hybrids ( CLASH ) can not only provide information about canonical but also non‐canonical interactions. We evaluated the associations between variants in micro RNA target sites using CLASH data and survival outcomes of 782 early‐stage non‐small cell lung cancer ( NSCLC ) patients who underwent curative surgical resection. Among the 100 variants studied, two variants showed significant association with survival outcomes. The POLR2A rs2071504 C > T variant was associated with poor overall and disease‐free survival under a dominant model (hazard ratio [ HR] 1.42, 95% confidence interval [ CI] 1.08–1.88; P = 0.01 and HR 1.34, 95% CI 1.08–1.67; P = 0.01, respectively). Patients carrying the NR2F6 rs2288539 TT genotype showed significantly better overall survival than those with the NR2F6 rs2288539 CC or CT genotypes ( HR 0.13, 95% CI 0.02–0.90; P = 0.04). These findings suggest that POLR2A rs2071504 C > T and NR2F6 rs2288539 C > T can influence prognosis in early‐stage NSCLC patients.