Open AccessExpression and clinical significance of A ‐kinase anchor protein 4 in lung adenocarcinoma tissue
Author(s) -
Li HongMei,
Guo Kang,
Yu XiaoYun,
Yu Zhuang,
Xu Ping
Publication year - 2016
Publication title -
thoracic cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 28
eISSN - 1759-7714
pISSN - 1759-7706
DOI - 10.1111/1759-7714.12324
Subject(s) - adenocarcinoma , lung , western blot , metastasis , messenger rna , medicine , cancer research , pathology , adenocarcinoma of the lung , biology , cancer , gene , biochemistry
Background The A‐kinase anchor proteins ( AKAP ) are a growing family of scaffolding proteins involved in the occurrence, proliferation, and metastasis of tumors by controlling intracellular signals. In this study, the expression and significance of AKAP 4 were analyzed in patients with lung adenocarcinoma and adjacent non‐cancerous tissues. Methods Using reverse transcriptase‐polymerase chain reaction and Western blot, AKAP 4 messenger ribonucleic acid (m RNA ) and protein expression levels were measured in 108 cases of lung adenocarcinoma and adjacent non‐cancerous tissues. Results AKAP 4 m RNA and protein were expressed in lung adenocarcinoma tissues, but not in adjacent non‐cancerous tissues. The expression of AKAP 4 m RNA and protein was closely associated with lymphatic metastasis ( P < 0.05), but had no relationship with stage, differentiation degree, gender, age or smoking ( P > 0.05). AKAP 4 expression had an adverse effect on the overall survival rate ( P < 0.05). Conclusion The expression of AKAP 4 was high in lung adenocarcinoma tissue, which may be closely related to the lymphatic metastasis of lung adenocarcinoma. AKAP 4 may be a novel lung adenocarcinoma molecule marker and a predictor of poor prognosis.