Open AccessMicro ribonucleic acid‐93 promotes proliferation and migration of esophageal squamous cell carcinoma by targeting disabled 2
Author(s) -
Li Chang,
Ding Cheng,
Chen Tengfei,
Chen Jun,
Xu Zhenlei,
Lei Zhe,
Xu Chun,
Zhao Jun
Publication year - 2015
Publication title -
thoracic cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 28
eISSN - 1759-7714
pISSN - 1759-7706
DOI - 10.1111/1759-7714.12242
Subject(s) - cell growth , cancer research , downregulation and upregulation , gene knockdown , transfection , microrna , cell cycle , flow cytometry , cell , cell migration , small interfering rna , medicine , cell culture , microbiology and biotechnology , biology , gene , biochemistry , genetics
Background Accumulated evidence has revealed that the dysregulation of micro ribonucleic acids (mi RNA s) may contribute to esophageal squamous cell carcinoma ( ESCC ). Mi R ‐93, which is a member of the mi RNA cluster mi R ‐106b∼25, has been widely studied for its tumor promoting effect on different types of cancers. However, our knowledge of mi R ‐93 function in ESCC remains unclear. Methods The expression levels of mi R ‐93 in ESCC and the adjacent non‐tumor tissues were measured by real‐time polymerase chain reaction. Cell counting kit‐8, flow cytometry, and 5‐ethynyl‐2′‐deoxyuridine incorporation and transwell migration assays were employed to explore the effects of miR‐93 on proliferation and migration capabilities in EC109 cells. To determine the possible target gene of mi R ‐93, cell transfection, Western blot analysis and luciferase reporter gene assays were performed. Results A significant upregulation of mi R ‐93 expression in ESCC tissues was determined, combined with a downregulation of the predicted target gene, disabled 2 ( DAB2) . The introduction of mi R ‐93 significantly promotes cell proliferation, cell cycle progression, and the metastatic capability of EC109 cells. By cell transfection and luciferase reporter assay, DAB2 was confirmed as a direct target of mi R ‐93. In addition, the knockdown of DAB2 by small interfering RNA displayed a consentaneous phenocopy with miR‐93 overexpression in EC109 cells. Conclusion Our results indicate that mi R ‐93 acts as a tumor promoter in ESCC , and its promotion effects on ESCC cell proliferation and migration depend largely upon DAB2 suppression.