Open AccessPotential anti‐cancer effect of curcumin in human lung squamous cell carcinoma
Author(s) -
Zhao Wei,
Wang Yan,
Wang Ying,
Gao Nan,
Han Zhifeng,
Yu Haixiang
Publication year - 2015
Publication title -
thoracic cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 28
eISSN - 1759-7714
pISSN - 1759-7706
DOI - 10.1111/1759-7714.12222
Subject(s) - viability assay , mapk/erk pathway , protein kinase b , cancer research , curcumin , medicine , vascular endothelial growth factor , signal transduction , mtt assay , microbiology and biotechnology , cell growth , biology , cell , pharmacology , biochemistry , vegf receptors
Background To explore the molecular mechanisms of the anti‐cancer effect of curcumin in human lung squamous cell carcinoma ( LSQCC ) SK‐MES ‐1 cells. Methods Cell viability was determined using MTT assay. Ribonucleic acid sequencing was performed to measure expression levels of transcripts in LSQCC cells treated with 15 μmol/L curcumin (treatment groups) or an equal amount of dimethylsulfoxide (control). Cuffdiff software was used to identify differentially expressed genes ( DEGs ) in treatment groups, followed by enrichment analysis of DEGs using the D atabase for A nnotation, V isualization and I ntegration D iscovery. The protein‐protein interaction ( PPI ) networks for up and downregulated DEGs were constructed by C ytoscape software using S earch T ool for the R etrieval of I nteracting G enes data to identify hub nodes. Results Curcumin significantly reduced cell viability in LSQCC cells. In total, 380 DEGs including 154 upregulated and 126 downregulated genes were found in the treatment groups. The upregulated genes were enriched in base excision repair ( BER , such as PCNA , POLL , and MUTYH ) and J anus kinase‐signal transducer and activator of transcription ( JAT‐STAT) signaling pathways (such as AKT 1 and STAT 5 A ), while the downregulated genes were enriched in nine pathways, including the vascular endothelial growth factor ( VEGF) signaling pathway (such as PTK 2 , VEGFA , MAPK 1 , and MAPK 14 ) and mitogen‐activated protein kinase ( MAPK) signaling pathway ( ARRB 2 , MAPK 1, MAPK 14 , and NFKB 1 ). PCNA and AKT 1 were the hub nodes in the PPI network of upregulated genes while MAPK 1 , MAPK 14 , VEGFA , and NFKB 1 were the hub nodes in the PPI network of downregulated genes. Conclusions Curcumin might exert anti‐cancer effects on LSQCC via regulating BER , JAT‐STAT , VEGF, and MAPK signaling pathways.